Circulating levels of Bcl-2 and its expression in the nasal mucosa of patients with chronic rhinosinusitis

Iuliia Kalashnyk-Vakulenko 1, Galina Gubina-Vakulyck 2, Anatolii Onishchenko 3, Oksana Nakonechna 4, Tatyana Gorbach 4, Viktoriya Tkachenko 5, Anton Tkachenko 3 *
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1 Department of Otorhinolaryngology, Kharkiv National Medical University, Kharkiv, Ukraine
2 Department of Pathological Anatomy, Kharkiv National Medical University, Kharkiv, Ukraine
3 Research Institute of Experimental and Clinical Medicine, Kharkiv National Medical University, Kharkiv, Ukraine
4 Department of Biochemistry, Kharkiv National Medical University, Kharkiv, Ukraine
5 Department of Microbiology, Virology and Immunology, Kharkiv National Medical University, Kharkiv, Ukraine
* Corresponding Author
J CLIN MED KAZ, Volume 19, Issue 6, pp. 63-67. https://doi.org/10.23950/jcmk/12689
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ABSTRACT

Aim: To evaluate expression of anti-apoptotic Bcl-2 protein in the nasal tissue and its levels in blood serum of patients with chronic rhinosinusitis with (CRSwNP) and without nasal polyps (CRSsNP).
Material and methods: Expression of Bcl-2 in the sinonasal tissue and its levels in blood serum of patients with CRSsNP and CRSwNP were evaluated immunohistochemically and using ELISA, respectively.
Results: In patients with CRSsNP, Bcl-2 was overexpressed in nasal epithelial cells mainly in the atrophic regions. However, its upregulation was also observed in regions with epithelial cell proliferation. Immunostaining for Bcl-2 was stronger both in the stroma and epithelial lining compared with control subjects. The level of Bcl-2 in blood serum was elevated in both forms of chronic rhinosinusitis with a more pronounced increase in CRSwNP.
Conclusion: CRSsNP and especially CRSwNP are associated with overexpression of anti-apoptotic Bcl-2 in nasal epithelial cells and in the lamina propria against the background of elevated circulating concentrations of Bcl-2.
Keywords: chronic rhinosinusitis, nasal polyps, nasal epithelial cells, apoptosis, B-cell lymphoma 2, Bcl-2.

CITATION

Kalashnyk-Vakulenko I, Gubina-Vakulyck G, Onishchenko A, Nakonechna O, Gorbach T, Tkachenko V, et al. Circulating levels of Bcl-2 and its expression in the nasal mucosa of patients with chronic rhinosinusitis. J CLIN MED KAZ. 2022;19(6):63-7. https://doi.org/10.23950/jcmk/12689

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