Detection of Circulating Tumor cells by ISET and their molecular characterization for use as liquid biopsy

Диагностика циркулирующих опухолевых клеток с помощью технологии ISET и их молекулярная характеристика для жидкостной биопсии
Gulziya Ismailova 1, Sophie Laget 2, Patrizia Paterlini-Bréchot 3
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1 JSC “National Scientific Medical Research Center”, Astana, Kazakhstan
2 INSERM/Rarecells Diagnostics team, Paris, France
3 University Paris Descartes, INSERM, Paris, France
J CLIN MED KAZ, Volume 1, Issue 35, pp. 15-20.
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ABSTRACT

Currently, the detection of theranostic biomarkers is performed on samples of tumor tissue, after pathological analysis. Circulating Tumor Cells (CTC) are a non-invasive source of tumor material potentially highly relevant to guide therapeutic choices and for early detection of resistance to therapy. The ISET (Isolation by Size of Tumor / Trophoblastic cells) method allows cytopathological diagnosis of CTC and their molecular analysis for theranostic analysis aimed to guide the use of targeted therapies. In particular, ISET allows the isolation of CTC without loss and without bias of selection and their immune-molecular characterization, including detection of vimentin, indicating indication their increased metastatic potential, and extensive analysis of gene mutations, such as EGFR, KRAS, ALK, HER2 genes, predictive of response to therapy.
Keywords: circulating tumor cells, ISET, cytopathology, oncogenetics

CITATION

Ismailova G, Laget S, Paterlini-Bréchot P. Detection of Circulating Tumor cells by ISET and their molecular characterization for use as liquid biopsy. Journal of Clinical Medicine of Kazakhstan. 2015;1(35):15-20.

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