Investigation of Microbiological Effects of Atmospheric Pressure Cold Plasma on Hand Disinfection

Şeyma TOY 1 * , Yusuf Secgin 1, Ferhat Bozduman 2, Şerife Yılmaz 3, Hasan Solmaz 3, Zulal Oner 4
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1 Department of Anatomy, Karabük University Faculty of Medicine, Karabük, Turkey
2 Department of Biophysics, Karabük University Faculty of Medicine, Karabük, Turkey
3 Department of Microbiology, Karabük University Faculty of Medicine, Karabük
4 Department of Anatomy, İzmir Bakırçay University Faculty of Medicine, İzmir, Turkey
* Corresponding Author
J CLIN MED KAZ, Volume 21, Issue 5, pp. 35-39. https://doi.org/10.23950/jcmk/15489
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Author Contributions: Conceptualization, Y. S., S. T., F. B., S. Y. and H. S.; methodology, Y. S., S. T., F. B., S. Y. and H. S.; validation, Y. S., S. T., Z. O., F. B., S. Y. and H. S.; formal analysis, Y. S., S. T., Z. O., F. B., S. Y. and H. S.; investigation, Y. S., S. T., Z. O., F. B., S. Y. and H. S.; resources, Y. S., S. T., Z. O., F. B., S. Y. and H. S.; data curation, Y. S., S. T., Z. O., F. B., S. Y. and H. S.; writing – original draft preparation, Y. S., S. T., Z. O., F. B., S. Y and H. S.; writing – review and editing, Y. S., S. T., Z. O., F. B., S. Y. and H. S.; visualization, Y. S., S. T., Z. O., F. B., S. Y. and H. S.; supervision, S. T.; project administration, S. T.; funding acquisition, S. T. All authors have read and agreed to the published version of the manuscript.

Statement of Ethics: The study was carried out with the decision of the local ethics committee of Karabük University dated 28.09.2022 and numbered 718. The study was carried out in accordance with the Declaration of Helsinki.

Informed Consent: A voluntary consent form was obtained.

ABSTRACT

Aim: Atmospheric pressure cold plasma is the fourth state of matter obtained at low temperature with atmospheric pressure, which has antimicrobial, anti-inflammatory, sterilization, disinfection and surface modification enhancing properties. The aim of this study is to investigate the effectiveness of plasma activated medium (PAM) obtained from atmospheric pressure cold plasma on hand disinfection.
Methods and Materials: The study was carried out on 15 individuals aged between 18 and 65 with healthy hand structures (no wounds, no pathology). Of the 15 individuals, 5 were selected as cleaning staff, 5 as office stuff, and 5 as academics. Samples were first taken from individuals using swap for bacterial identification. Afterwards, they were asked to wash their hands for 5 minutes in 250 ml PAM. At the end of the period, samples were taken from the individuals in the same way for bacterial identification using swap. The effectiveness of PAM in terms of hand disinfection was determined by comparing these two samples.
Results: As a result of our study, it was found that bacterial colonies decreased in 3 of the samples taken from the hands of 5 cleaning personnel, remained stable in 2, a decrease in bacterial colonies in 4 of 5 office stuff, remained constant in 1, a decrease in bacterial colonies in 3 of 5 academicians, and remained stable in 2 of them. Staphylococcus aureus, Staphylococcus epidermidis, Bacillus megaterium, Staphylococcus capitis, Streptococcus mitis, Staphylococcus warneri bacteria were identified on the hands of individuals.
Conclusion: As a result of our study, it was found that PAM, which has no side effects in the literature, can be an effective tool for hand disinfection.
Keywords: Plasma, Hand disinfection, Microbiological Efficacy, Plasma activated environment

CITATION

TOY Ş, Secgin Y, Bozduman F, Yılmaz Ş, Solmaz H, Oner Z. Investigation of Microbiological Effects of Atmospheric Pressure Cold Plasma on Hand Disinfection. J CLIN MED KAZ. 2024;21(5):35-9. https://doi.org/10.23950/jcmk/15489

REFERENCES

  • Laroussi M. Nonthermal decontamination of biological media by atmospheric-pressure plasmas: review, analysis, and prospects. IEEE Transactions on plasma science. 2002; 30(4): 1409–1415. https://doi.org/10.1109/TPS.2002.804220.
  • Moisan M, Barbeau J, Crevier M-C, Pelletier J, Philip N, Saoudi B. Plasma sterilization. Methods and mechanisms. Pure and applied chemistry. 2002; 74(3): 349–358. https://doi.org/10.1351/pac200274030349.
  • Cha S, Park Y-S. Plasma in dentistry. Clinical plasma medicine. 2014; 2(1): 4–10. https://doi.org/10.1016/j.cpme.2014.04.002.
  • Küçük D, Ercan UK, Köseoğlu S. Maddenin dördüncü hali: Plazma ve atmosferik basınçlı soğuk plazmaların diş hekimliğinde kullanımı. 7tepe Klinik. 2018; 14(3): 125–136. https://doi.org/10.5505/yeditepe.2018.09609.
  • Hoffmann C, Berganza C, Zhang J. Cold Atmospheric Plasma: methods of production and application in dentistry and oncology. Medical gas research. 2013; 3(1): 1–15. https://doi.org/10.1186/2045-9912-3-21.
  • Akçalı K, Oktav Bulut M. Plazma teknolojilerinin yün elyafı üzerindeki etkileri üzerine bir inceleme. Journal of Engineering Science and Design. 2012: 2 (1): 65–72.
  • Vecchio D, Dai T, Huang L, Fantetti L, Roncucci G, Hamblin MR. Antimicrobial photodynamic therapy with RLP068 kills methicillin‐resistant Staphylococcus aureus and improves wound healing in a mouse model of infected skin abrasion PDT with RLP068/Cl in infected mouse skin abrasion. Journal of biophotonics. 2013; 6(9): 733-742. https://doi.org/10.1002/jbio.201200121.
  • Li R, Ye L, Mai Y-W. Application of plasma technologies in fibre-reinforced polymer composites: a review of recent developments. Composites Part A: Applied Science and Manufacturing. 1997; 28(1): 73–86. https://doi.org/10.1016/S1359-835X(96)00097-8.
  • Bozkurt D. Soğuk Plazma Uygulamasının Vitaminler Ve Polifenol Oksidaz (Pfo) Enzimi Aktivitesi Üzerine Etkisi. Fen bilimleri Enstitüsü. 2014.
  • Tušek L, Nitschke M, Werner C, Stana-Kleinschek K, Ribitsch V. Surface characterisation of NH3 plasma treated polyamide 6 foils. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2001; 195(1–3): 81–95. https://doi.org/10.1016/S0927-7757(01)00831-7.
  • Oztan MO, Ercan UK, Aksoy Gokmen A, Simsek F, Ozdemir GD, Koyluoglu G. Irrigation of peritoneal cavity with cold atmospheric plasma treated solution effectively reduces microbial load in rat acute peritonitis model. Scientific Reports. 2022; 12(1): 1–15. https://doi.org/10.1038/s41598-022-07598-2.
  • Cheng Y-J, Lin C-K, Chen C-Y, Chien P-C, Chuan H-H, Ho C-C, et al. Plasma-activated medium as adjuvant therapy for lung cancer with malignant pleural effusion. Scientific reports. 2020; 10(1): 1–15. https://doi.org/10.1038/s41598-020-75214-2.
  • Isbary G, Shimizu T, Li Y-F, Stolz W, Thomas HM, Morfill GE, et al. Cold atmospheric plasma devices for medical issues. Expert review of medical devices. 2013; 10(3): 367–377. https://doi.org/10.1586/erd.13.4.
  • Ermolaeva SA, Varfolomeev AF, Chernukha MY, Yurov DS, Vasiliev MM, Kaminskaya AA, et al. Bactericidal effects of non-thermal argon plasma in vitro, in biofilms and in the animal model of infected wounds. Journal of medical microbiology. 2011; 60(1): 75–83. https://doi.org/10.1099/jmm.0.020263-0.
  • Bernhardt T, Semmler ML, Schäfer M, Bekeschus S, Emmert S, Boeckmann L. Plasma medicine: Applications of cold atmospheric pressure plasma in dermatology. Oxidative medicine and cellular longevity. 2019; 2019. https://doi.org/10.1155/2019/3873928.
  • Çoban E, Arslan G, Keven F, Ayhan F, Ayhan H. Atmosferik Plazma Teknolojisi Kullanılarak Cep Telefonu Koruma Malzemelerine Antimikrobiyal Özellik Kazandırılması. Avrupa Bilim ve Teknoloji Dergisi. 2021; (21): 53–66. https://doi.org/10.31590/ejosat.798436.
  • Attri P, Park JH, Ali A, Choi EH. How does plasma activated media treatment differ from direct cold plasma treatment? Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Anti-Cancer Agents). 2018; 18(6): 805–814. https://doi.org/10.2174/1871520618666180406121734.
  • Keidar M, Walk R, Shashurin A, Srinivasan P, Sandler A, Dasgupta S, et al. Cold plasma selectivity and the possibility of a paradigm shift in cancer therapy. British journal of cancer. 2011; 105(9): 1295–1301. https://doi.org/10.1038/bjc.2011.386.
  • Özdemir A. Soğuk Atmosferik Plazma ve Kanser. Researcher. 2021; 1(02): 6–18.
  • Daeschlein G, Scholz S, Arnold A, von Podewils S, Haase H, Emmert S, et al. In vitro susceptibility of important skin and wound pathogens against low temperature atmospheric pressure plasma jet (APPJ) and dielectric barrier discharge plasma (DBD). Plasma Processes and Polymers. 2012; 9(4): 380–389. https://doi.org/10.1002/ppap.201100160.
  • Daeschlein G, Scholz S, von Woedtke T, Niggemeier M, Kindel E, Brandenburg R, et al. In vitro killing of clinical fungal strains by low-temperature atmospheric-pressure plasma jet. IEEE Transactions on Plasma Science. 2010; 39(2): 815–821. https://doi.org/10.1109/TPS.2010.2063441.
  • Isbary G, Shimizu T, Zimmermann J, Heinlin J, Al-Zaabi S, Rechfeld M, et al. Randomized placebo-controlled clinical trial showed cold atmospheric argon plasma relieved acute pain and accelerated healing in herpes zoster. Clinical Plasma Medicine. 2014; 2(2): 50–55. https://doi.org/10.1016/j.cpme.2014.07.001.
  • Arman D. El yıkama ve el dezenfeksiyonu. Hastane İnfeksiyonları Dergisi. 2003; 7: 76–82.
  • Günaydın M. Hastane Enfeksiyonları ve El Hijyeni. 24 DAS Eğitim Semineri, 15 Haziran 2013, Karaman.
  • Erol S. El Antisepsisi, Cerrahi El Antisepsisi El Hijyeninde Kullanılan Solüsyonlar ve Yumuşatıcılar. 6. Ulusal Sterilizasyon Dezenfeksiyon Kongresi. 2009; 1(5): 443–453.
  • Flora, Eldeki normal bakteriyel. El Yıkama ve El Dezenfeksiyonu.
  • Bulut ME, Öncül A. Nosocomial infection agents of Şişli Hamidiye Etfal training and research hospital: Comparison of 1995 and 2017 data. The Medical Bulletin of Sisli Etfal Hospital. 2020; 54(1): 78. https://doi.org/10.14744/SEMB.2019.03271.
  • Çopur B. El yıkama çeşitleri ve dikkat edilecek hususlar. 4. Ulusal Sterilizasyon Dezenfeksiyon Kongresi. 2005: 20–24.