Investigation of Microbiological Effects of Atmospheric Pressure Cold Plasma on Hand Disinfection

Şeyma TOY 1 * , Yusuf Secgin 1, Ferhat Bozduman 2, Şerife Yılmaz 3, Hasan Solmaz 3, Zulal Oner 4
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1 Department of Anatomy, Karabük University Faculty of Medicine, Karabük, Turkey
2 Department of Biophysics, Karabük University Faculty of Medicine, Karabük, Turkey
3 Department of Microbiology, Karabük University Faculty of Medicine, Karabük
4 Department of Anatomy, İzmir Bakırçay University Faculty of Medicine, İzmir, Turkey
* Corresponding Author
J CLIN MED KAZ, Volume 21, Issue 5, pp. 35-39. https://doi.org/10.23950/jcmk/15489
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Author Contributions: Conceptualization, Y. S., S. T., F. B., S. Y. and H. S.; methodology, Y. S., S. T., F. B., S. Y. and H. S.; validation, Y. S., S. T., Z. O., F. B., S. Y. and H. S.; formal analysis, Y. S., S. T., Z. O., F. B., S. Y. and H. S.; investigation, Y. S., S. T., Z. O., F. B., S. Y. and H. S.; resources, Y. S., S. T., Z. O., F. B., S. Y. and H. S.; data curation, Y. S., S. T., Z. O., F. B., S. Y. and H. S.; writing – original draft preparation, Y. S., S. T., Z. O., F. B., S. Y and H. S.; writing – review and editing, Y. S., S. T., Z. O., F. B., S. Y. and H. S.; visualization, Y. S., S. T., Z. O., F. B., S. Y. and H. S.; supervision, S. T.; project administration, S. T.; funding acquisition, S. T. All authors have read and agreed to the published version of the manuscript.

Statement of Ethics: The study was carried out with the decision of the local ethics committee of Karabük University dated 28.09.2022 and numbered 718. The study was carried out in accordance with the Declaration of Helsinki.

Informed Consent: A voluntary consent form was obtained.

ABSTRACT

Aim: Atmospheric pressure cold plasma is the fourth state of matter obtained at low temperature with atmospheric pressure, which has antimicrobial, anti-inflammatory, sterilization, disinfection and surface modification enhancing properties. The aim of this study is to investigate the effectiveness of plasma activated medium (PAM) obtained from atmospheric pressure cold plasma on hand disinfection.
Methods and Materials: The study was carried out on 15 individuals aged between 18 and 65 with healthy hand structures (no wounds, no pathology). Of the 15 individuals, 5 were selected as cleaning staff, 5 as office stuff, and 5 as academics. Samples were first taken from individuals using swap for bacterial identification. Afterwards, they were asked to wash their hands for 5 minutes in 250 ml PAM. At the end of the period, samples were taken from the individuals in the same way for bacterial identification using swap. The effectiveness of PAM in terms of hand disinfection was determined by comparing these two samples.
Results: As a result of our study, it was found that bacterial colonies decreased in 3 of the samples taken from the hands of 5 cleaning personnel, remained stable in 2, a decrease in bacterial colonies in 4 of 5 office stuff, remained constant in 1, a decrease in bacterial colonies in 3 of 5 academicians, and remained stable in 2 of them. Staphylococcus aureus, Staphylococcus epidermidis, Bacillus megaterium, Staphylococcus capitis, Streptococcus mitis, Staphylococcus warneri bacteria were identified on the hands of individuals.
Conclusion: As a result of our study, it was found that PAM, which has no side effects in the literature, can be an effective tool for hand disinfection.
Keywords: Plasma, Hand disinfection, Microbiological Efficacy, Plasma activated environment

CITATION

TOY Ş, Secgin Y, Bozduman F, Yılmaz Ş, Solmaz H, Oner Z. Investigation of Microbiological Effects of Atmospheric Pressure Cold Plasma on Hand Disinfection. J CLIN MED KAZ. 2024;21(5):35-9. https://doi.org/10.23950/jcmk/15489

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