Monitoring of intestinal microbiocenosis of children under 15 years

Мониторинг кишечного микробиоценоза у детей до 15 лет
Nelya Bissenova 1, Aigerim Yergaliyeva 1, Aigul Kuanyshbekova 1, Marina Sitnikova 1
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1 JSC «National Scientific Medical Center», Astana, Kazakhstan
J CLIN MED KAZ, Volume 2, Issue 36, pp. 30-33.
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ABSTRACT

The aim of this study: the monitoring of intestinal microbiocenosis of children under 15 years.
Methods: During 2012 - 2014 conducted prospective bacteriological examination of the large intestine 255 children under 15 years. Primary inoculation of clinical material was performed quantitative method to culture media in accordance with the regulations. The identification of isolates was performed by microbiological analyzer Vitek 2-Compact.
Results: From 2012 to 2014 bacteriological examination for intestinal dysbiosis characterized by a decrease the number of obligate microflora. Escherichia coli with normal enzymatic activity normally have been allocated 59.6±3.0% of the surveyed children, bifidobacteria were detected at 62.3±3.0%, only 16.0±2.3% of infants were identified in normal lactobacilli. The study showed a detection of opportunistic enterobacteria in 21.5±2.5% of patients (in particular bacteria of the genus Proteus, Klebsiella, Enterobacter), Candida in 18.8±2.4%, Staphylococcus aureus - 16.0±2.3%.
Conclusions: Intestinal microflora of infants is characterized by a decrease in the number of lactobacilli, bifidobacteria and E.coli with normal enzymatic activity. As part of intestinal microbiocenosis of children surveyed increased the number of opportunistic enterobacteria, Candida and Clostridium.
Keywords: intestinal dysbiosis in children, bifidobacteria, lactobacilli, opportunistic enterobacteria

CITATION

Bissenova N, Yergaliyeva A, Kuanyshbekova A, Sitnikova M. Monitoring of intestinal microbiocenosis of children under 15 years. Journal of Clinical Medicine of Kazakhstan. 2015;2(36):30-3.

REFERENCES

  • Kipshakbaev R.K., Kipshakbaeva F.M. Obzor problem disbioza kishechnika u detej (Overview of problems of intestinal dysbiosis in children), Zhurnal Chelovek i lekarstvo, 2014, No.7, pp. 2-5.
  • Denisov M.Ju. Disbioticheskie narushenija kishechnika u detej (Dysbiotic bowel disorders in children), Pediatricheskaja farmakologija, 2011, No.5, pp.54-60.
  • Otraslevoj standart RF «Protokol vedenija bol’nyh. Disbakterioz kishechnika» (Industry Standard of the Russian Federation “Treatment Protocol. Intestinal dysbiosis”), Moskva, 2003, pp.10-14.
  • Nemchenko U.M., Popkova S.M., Dzhioev Ju.P. i dr. Vidovoe raznoobrazie bifidobakterij u detej s funkcional’nymi zheludochno-kishechnogo trakta (Species diversity bifidobacteria in children with functional gastrointestinal), Bjulleten’ Vostochno-Sibirskogo nauchnogo centra RAMN, 2012, No.2, pp.50-54.
  • Bisenova N.M., Mitus N.M., Tuleubaeva Je.A. i dr. Rol’ uslovno-patogennyh jenterobakterij v razvitii disbakterioza kishechnika u detej (The role of opportunistic enterobacteria in the development of intestinal dysbiosis in children), Klinicheskaja medicina Kazahstana, 2011, No.3-4, pp.343-344.
  • Batchaev H.H., Arapova V.I., Pilipenko T.D. Harakteristika narushenij kishechnoj mikrobioty (Characteristics of violations of the intestinal microbiota), ZhMJeI, 2010, No.2, pp.99-101.
  • Standarty vzjatija, dostavki i hranenija biomateriala dlja mikrobiologicheskih issledovanij v laboratorii klinicheskoj mikrobiologii (Standards capture, delivery and storage of biological material for microbiological studies in the clinical microbiology laboratory), Metodicheskie instrukcii, Astana, 2008, pp.11-12.
  • Bakteriologicheskaja diagnostika disbakterioza kishechnika (The bacteriological diagnosis of intestinal dysbiosis), Metodicheskie ukazanija № 10.05.044.03, Almaty, 2003, pp.8-18.
  • Opredelitel’ bakterij Berdzhi (The determinant of bacteria Burgi). Pod. red. Dzh. Houlta. M.:Mir, 1997, T.1., pp.180-196.
  • Kotova A.L. Normoflora i disbakteriozy cheloveka (Normal flora and human dysbacterioses), Almaty, 2008, pp.331-340.
  • Suvorova M.A., Rjabchuk F.N. Chuvstvitel’nost’ mikrobioty kishechnika k bakteriofagam i probiotikam u detej s zabolevanijami organov pishhevarenija (The sensitivity of the intestinal microbiota to bacteriophages and probiotics in children with digestive diseases), Lechashhij vrach, 2011, No.6, pp. 14-16.
  • Bisenova N.M., Ergalieva A.S. Fakul’tativnaja mikroflora kishechnika u detej pervogo goda zhizni (Optional intestinal microflora in infants), Astana Medicinalyk Zhurnaly, 2011, No.2, pp.33-35.