Prediction and diagnostics of endometriosis in women with infertility based on immunological markers

Прогнозирование и диагностика эндометриоза у женщин с бесплодием на основании иммунологических маркеров
Halyna Koval 1, Maria Ivanchuk 1
More Detail
1 Bukovinian State Medical University, Chernivtsi, Ukraine
J CLIN MED KAZ, Volume 2, Issue 36, pp. 23-29.
OPEN ACCESS 3055 Views 2202 Downloads
Download Full Text (PDF)

ABSTRACT

The aim of this study: а determination of cytokines informative for predicting and diagnostics of the presence of endometriosis in women with infertility.
Methods: The main groups of 65 women with infertility associated with endometriosis have been researched as well as control group of 40 women with infertility of tubal genesis. The pre-defined levels of several cytokines (IL-2, INF-γ, TNF-α, IL-6, IL-10, TGF-β, IL-17, IL-1β, IL-12, IL-18) in serum and peritoneal fluid. Identification of immunological markers informative for predicting and diagnostics of the presence of endometriosis in women with infertility was conducted using mathematical models for informative by Kulbaka, Valdivsky sequential analysis, cluster analysis and linear separation method of convex hulls. The indices for prediction were selected according to Uilkokson-Mann-Whitney criterion and have showed probable differences (p<0.05) between the groups of patients with the presence and absence of endometriosis.
Results: the cytokines of peritoneal fluid has bigger value, than the cytokines of serum for predicting and diagnostics of the presence of endometriosis. But bigger practical value has the cytokines of serum, because collecting of peritoneal fluid is more invasions. The highest informative for endometriosis prediction was demonstrated by interleukin 6 and tumor necrosis factor-α cytokines.
Conclusion: IL-6 and TNF-α of serum can be used as immunological markers for predicting and diagnostics of the presence of endometriosis in women with infertility.
Keywords: endometriosis, infertility, forecasting, diagnostics, cytokines

CITATION

Koval H, Ivanchuk M. Prediction and diagnostics of endometriosis in women with infertility based on immunological markers. Journal of Clinical Medicine of Kazakhstan. 2015;2(36):23-9.

REFERENCES

  • Baskakov V.P., Cvelev Ju.V., Kira E.F. Endometrioid disease, SPb, 2002, 452 p.
  • Marchenko L.A., Il’ina L.M. A modern look at some aspects of the pathogenesis of endometriosis (review), Problemy reprodukcii, 2011, No.1, pp. 20-25.
  • Adamjan L.V., Kulakov V.I., Andreeva E.N. Endometriosis. Guide for physicians, M.: Medicina, 2006,170 p.
  • Savickij G.A., S.M. Gorbushin. Peritoneal endometriosis and infertility (clinical and morphological studies), SPb.: JeLBISPb, 2002, 170 p.
  • Giudice L.C., Kao L.C. Endometriosis, Lancet, 2004, No.364, pp.1789–1799.
  • Ozerskaja A. Sonography in Gynecology, Medika, Moskva,2005, pp.112 – 113.
  • Shimanovskij N.L. Application of MRI with contrast enhancement in the diagnosis of endometriosis, Mezhdunarodnyj medicinskij zhurnal, 2005, T. 11, No. 3, pp. 73-76.
  • Wu T.T., Coakley F.V., Qayyum A. et al. Magnetic resonance imaging of ovarian cancer arising in endometriomas, J. Comp. Assist. Tomogr, 2004, No. 28, pp.836–838.
  • Balleyguier C., Roupret M., Nguyen T. et al. Ureteral endometriosis: the role of magnetic resonance imaging, J. Am. Assoc. Gynecol. Laparosc, 2004, No.4, pp. 530–536.
  • Catherine B., Wykes T., Justin Clark et al. Review: Accuracy of laparoscopy in the diagnosis of endometriosis: a systematic quantitative review, BJOG: An Intern. Journal of Obstetrics & Gynaecology, 2004,Vol. 111,No. 11, pp. 1204–1212.
  • Cochrane Methods Working Group on Systematic Reviews of Screening and Diagnostic Tests. Recommended Methods. 1996. Available: http://www.cochrane.org/cochrane/sadtdoc1.htm (Accessed 3 September 2001).
  • Zaporozhan V.M., Cegel’s’kij M.R. Gynecologic Pathology: Atlas: Textbook, Odesa: Odes. derzh. med. un-t, 2002, 30 p.
  • Howard F.M., Minawi E.I., Sanchez A.M. Conscious pain mapping by laparoscopy in women with chronic pelvic pain, Obstet Gynecol, 2000, No.96, pp. 934– 939.
  • Walter A.J., Hentz J.G., Magtibay P.M. et al. Endometriosis: correlation between histologic and visual findings at laparoscopy, Am J Obstet Gynecol, 2001, No.184, pp.1407–1411.
  • Ratcliffe N., Wright J.T., Importance of histological diagnosis in patients with pelvic pain and laparoscopic evidence of endometriosis, Gynaecol Endosc, 2000, No.9, pp. 301– 304.
  • Kontoravdis A., Hassan E., Hassiakos D. et al.. Laparoscopic evaluation and management of chronic pelvic pain during adolescence, Clin. Exp. Obstet. Gynecol, 1999, No. 26, pp. 76 - 77.
  • Mcleod B.S., Retzolff M.G.. Epidimiologi of endometriosis: an assessment of risk factors, Clin. Obstet. Gynecol, 2010, No. 53, pp. 39 – 396.
  • Burlev V.A., Il’jasova N.A., Dubinskaja E.D. et. al. Diagnostic value of markers of angiogenesis in biological fluids of patients with adenomyosis, Problemy reprodukcii, 2009, No1, pp.86-88.
  • Meola J., Júlio César Rosa e Silva, Blassioli D. et al. Differentially expressed genes in eutopic and ectopic endometrium of women with endometriosis, Fertility and Sterility,2010, No. 93 (6), pp.1750-1773.
  • Ding X., Wang L., Ren Y. Detection of mitochondrial biomarkers in eutopic endometria of endometriosis using surfaceenhanced laser desorption/ionization time-of-flight mass spectrometry, Fertility and Sterility, 2010, No. 94(7), pp. 2528-2530.
  • Kyama K.M., Overbergh L., Attila M. et. al. Endometrial and peritoneal expression of aromatase, cytokines, and adhesion factors in women with endometriosis, Fertility and Sterility, 2008, No. 89( 2), pp.301–310.
  • Ilie I., Ilie R. Cytokines and Endometriosis - the Role of Immunological Alterations, Biotechnology, molecular biology and nanomedicine, 2013, Vol.1, No.2, pp.8-18.
  • Khan K.N., Kitajima M., Hiraki K. et al. Immunopathogenesis of pelvic endometriosis: role of hepatocyte growth factor, macrophages and ovarian steroids, Am J Reprod Immunol. 2008, Vol.60, pp.383-404.
  • Barcz E., Milewski L., Dziunycz P. et al. Peritoneal cytokines and adhesion formation in endometriosis: an inverse association with vascular endothelial growth factor concentration, Fertility and sterility, 2012, No. 97, pp.1380-1386.
  • Kul’bak S. Information theory and statistics, M.: Mir, 1967, 408 p.
  • Gubler E. V., Genkin A. A. Application of nonparametric statistics in biomedical research, L., 1973, 144 p.
  • Mandel’ I.D. Cluster analysis, M.: Finansy i statistika, 1988, 176 p.
  • Hoppner Frank et al. Fuzzy Cluster Analysis. Methods for classification, data analysis and image recognition, John Wiley & Sons, LTD, 2000, 289 p.
  • Ivanchuk M.A., Malyk I.V. Separation of convex hulls as a way of modeling forecasting of complications in patients, Problemy upravlenija i informatiki, 2015, No.2, pp.89-95.
  • Koval’ G.D., Pashkovskaja N.V., Olenovich O.A. et. al. Features of systemic and local production of proinflammatory cytokines in women with endometriosis are infertile asotsiirovannym, The collection of materials of the International scientific conference “Modern research of biomedical sciences: improving the diagnosis, the development of prevention and treatment of diseases”, Russia, Kirov, 26-28 June, 2013, pp. 67-73.
  • Lakin G.F. Biometrics: A manual for biologists spices. universities, 4th ed., rev. and additional, M.: Vyssh. shkola, 1990, 352 p. 32.
  • Harada T., Yoshioka H., Yoshida S. et al. Increased interleukin-6 levels in peritoneal fluid of infertile patients with active endometriosis, American journal of obstetrics and gynecology, 1997, Vol. 176, pp.593-597.
  • Iwabe T., Harada T., Terakawa N. Role of cytokines in endometriosis-associated infertil-ity, Gynec. Obstet. Invest, 2002, No. 53, pp. 19-25.
  • Antsiferova Y.S., Sotnikova N.Y., Posiseeva L.V. et al. Changes in the T-helper cytokine profile and in lymphocyte activation at the systemic and local levels in women with endometriosis, Fertility and sterility, 2005, No. 84, pp.1705-1711.
  • Kalu E., Sumar N., Giannopoulos T. et al. Cytokine profiles in serum and peritoneal fluid from infertile women with and without endometriosis, The journal of obstetrics and gynaecology research, 2007, No.33, pp. 490-495.
  • Somigliana E., Viganò P., Candiani M. et al. Use of serum-soluble intercellular adhesion molecule-1 as a new marker of endometriosis, Fertility and Sterility, 2002, No. 77(5), pp.1028-1031.
  • Michaud N., Al-Akoum M., Gagnon G. et al. Decreased concentrations of soluble interleukin-1 receptor accessory protein levels in the peritoneal fluid of women with endometriosis, Journal of reproductive immunology, 2011, No. 92, pp. 68-73.