Reactive oxygen species (ROS) generation by lymphocytes in rats treated with a common food additive E407a

Anton Tkachenko 1 *
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1 Biochemistry Department, Kharkiv National Medical University, Kharkiv, Ukraine
* Corresponding Author
J CLIN MED KAZ, Volume 1, Issue 55, pp. 22-26. https://doi.org/10.23950/1812-2892-JCMK-00744
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ABSTRACT

This research deals with the evaluation of reactive oxygen species (ROS) generation in lymphocytes of rats orally exposed to a thickener and emulsifier E407a (semi-refined carrageenan). There is some evidence that E407a may be toxic and can induce intestinal inflammation. ROS generation in lymphocytes was assessed by flow cytometry using a dye 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) in 8 rats treated with 140 mg of semi-refined carrageenan per kg of weight daily during two weeks and 8 control animals. A higher number of ROShigh viable lymphocytes were found in the group of animals exposed to E407a compared with controls. In addition, mean fluorescence intensity (MFI) in ROShigh lymphocytes was elevated, which is indicative of the intensification of ROS production by lymphocytes in response to E407a oral consumption.
Keywords: carrageenan, food additive, reactive oxygen species, lymphocytes, inflammation

CITATION

Tkachenko A. Reactive oxygen species (ROS) generation by lymphocytes in rats treated with a common food additive E407a. Journal of Clinical Medicine of Kazakhstan. 2020;1(55):22-6. https://doi.org/10.23950/1812-2892-JCMK-00744

REFERENCES

  • Sudha PN, Aisverya S, Nithya R, Vijayalakshmi K. Industrial applications of marine carbohydrates. Adv Food Nutr Res. 2014; 73:145-81. https://doi.org/10.1016/B978-0-12-800268-1.00008-1
  • Campo VL, Kawano DF, da Silva DB, Carvalho I. Carrageenans: Biological properties, chemical modifications and structural analysis-A review. Carbohydrate Polymers. 2009; 77(2):167-180. https://doi.org/10.1016/j.carbpol.2009.01.020
  • Hotchkiss S, Brooks M, Campbell R, Philp K, Trius A. The use of carrageenan in food. In: Pereira L (ed) Carrageenans: sources and extraction methods, molecular structure, bioactive properties and health effects, 2016, 1st edn. Nova Science Publications Inc., New York. 1-293.
  • Diogo JV, Novo SG, González MJ, Ciancia M, Bratanich AC. Antiviral activity of lambda-carrageenan prepared from red seaweed (Gigartina skottsbergii) against BoHV-1 and SuHV-1. Res Vet Sci. 2015; 98:142-4. https://doi.org/10.1016/j.rvsc.2014.11.010
  • Besednova NN, Zvyagintseva TN, Kuznetsova TA, Makarenkova ID, Smolina TP, Fedyanina LN, et al. Marine algae metabolites as promising therapeutics for the prevention and treatment of HIV/AIDS. Metabolites. 2019; 9(5):87. https://doi.org/10.3390/metabo9050087
  • Luo M, Shao B, Nie W, Wei XW, Li YL, Wang BL, et al. Antitumor and adjuvant activity of λ-carrageenan by stimulating immune response in cancer immunotherapy. Sci Rep. 2015; 5:11062. https://doi.org/10.1038/srep11062
  • Morokutti-Kurz M, König-Schuster M, Koller C, Graf C, Graf P, Kirchoff N, et al. The intranasal application of Zanamivir and carrageenan is synergistically active against influenza a virus in the murine model. PLoS One. 2015; 10(6):e0128794. https://doi.org/10.1371/journal.pone.0128794
  • Leibbrandt A, Meier C, König-Schuster M, Weinmüllner R, Kalthoff D, Pflugfelder B, et al. Iota-carrageenan is a potent inhibitor of influenza A virus infection. PLoS One. 2010; 5(12):e14320. https://doi.org/10.1371/journal.pone.0014320
  • Calvo GH, Cosenza VA, Sáenz DA, Navarro DA, Stortz CA, Céspedes MA, et al. Disaccharides obtained from carrageenans as potential antitumor agents. Sci Rep. 2019; 9(1):6654. https://doi.org/10.1038/s41598-019-43238-y
  • McKim JM, Willoughby JA Sr, Blakemore WR, Weiner ML. Clarifying the confusion between poligeenan, degraded carrageenan, and carrageenan: A review of the chemistry, nomenclature, and in vivo toxicology by the oral route. Crit Rev Food Sci Nutr. 2019; 59(19):3054-3073. https://doi.org/10.1080/10408398.2018.1481822
  • Tkachenko A, Marakushyn D, Kalashnyk I, Korniyenko Y, Onishchenko A, Gorbach T, et al. A study of enterocyte membranes during activation of apoptotic processes in chronic carrageenan-induced gastroenterocolitis. Med Glas (Zenica). 2018; 15(2):87-92. https://doi.org/10.17392/946-18
  • Gubina-Vakyulyk GI, Gorbach TV, Tkachenko AS, Tkachenko MO. Damage and regeneration of small intestinal enterocytes under the influence of carrageenan induces chronic enteritis. Comparative Clinical Pathology. 2015; 24(6):1473-1477. https://doi.org/10.1007/s00580-015-2102-3
  • Necas J, Bartosikova L. Carrageenan: a review. Veterinarni Medicina 2013; 58:187-205. https://doi.org/10.17221/6758-VETMED
  • Tobacman JK. Review of harmful gastrointestinal effects of carrageenan in animal experiments. Environ Health Perspect. 2001; 109(10):983-994. https://doi.org/10.1289/ehp.01109983
  • Bhattacharyya S, Dudeja PK, Tobacman JK. Carrageenan-induced NFκB activation depends on distinct pathways mediated by reactive oxygen species and Hsp27 or by Bcl10. Biochimica et Biophysica Acta-General Subjects. 2008; 1780(7-8):973-982. https://doi.org/10.1016/j.bbagen.2008.03.019
  • Chen HM, Yan XJ, Mai TY, Wang F, Xu WF. Lambda-carrageenan oligosaccharides elicit reactive oxygen species production resulting in mitochondrial-dependent apoptosis in human umbilical vein endothelial cells. Int J Mol Med. 2009; 24(6):801-6. https://doi.org/10.3892/ijmm_00000295
  • Barth CR, Funchal GA, Luft C, de Oliveira JR, Porto BN, Donadio MV. Carrageenan-induced inflammation promotes ROS generation and neutrophil extracellular trap formation in a mouse model of peritonitis. Eur J Immunol. 2016; 46(4):964-70. https://doi.org/10.1002/eji.201545520
  • McKim JM Jr, Baas H, Rice GP, Willoughby JA Sr, Weiner ML, Blakemore W. Effects of carrageenan on cell permeability, cytotoxicity, and cytokine gene expression in human intestinal and hepatic cell lines. Food Chem Toxicol. 2016; 96:1-10. https://doi.org/10.1016/j.fct.2016.07.006
  • McKim JM. Food additive carrageenan: part I: A critical review of carrageenan in vitro, studies, potential pitfalls, and implications for human health and safety. Crit. Rev. Toxicol. 2014; 40:210-243. https://doi.org/10.3109/10408444.2013.861797
  • Abe T, Kawamura H, Kawabe S, Watanabe H, Gejyo F, Abo T. Liver injury due to sequential activation of natural killer cells and natural killer T cells by carrageenan. J Hepatol. 2002; 36(5):614-23. https://doi.org/10.1016/S0168-8278(02)00022-3
  • Frossard CP, Hauser C, Eigenmann PA. Oral carrageenan induces antigen-dependent oral tolerance: prevention of anaphylaxis and induction of lymphocyte anergy in a murine model of food allergy. Pediatr Res. 2001; 49(3):417-22. https://doi.org/10.1203/00006450-200103000-00018
  • Tkachenko AS, Onishchenko AI, Lesovoy VN, Myasoedov VV. Common food additive E407a affects BCL-2 expression in lymphocytes in vitro. Studia Univ. VG, SSV. 2019; 29(4):169-76.
  • Yarosz EL, Chang CH. The role of reactive oxygen species in regulating T cell-mediated immunity and disease. Immune Netw. 2018; 18(1):e14. https://doi.org/10.4110/in.2018.18.e14
  • Belikov AV, Schraven B, Simeoni L. T cells and reactive oxygen species. J Biomed Sci. 2015; 22:85. https://doi.org/10.1186/s12929-015-0194-3 
  • Sizzano F, Collino S, Cominetti O, Monti D, Garagnani P, Ostan R, et al. Evaluation of lymphocyte response to the induced oxidative stress in a cohort of ageing subjects, including semisupercentenarians and their offspring. Mediators Inflamm. 2018; 2018:7109312. https://doi.org/10.1155/2018/7109312
  • Amico D, Spadoni T, Rovinelli M, Serafini M, D'Amico G, Campelli N, et al. Intracellular free radical production by peripheral blood T lymphocytes from patients with systemic sclerosis: role of NADPH oxidase and ERK1/2. Arthritis Res Ther. 2015; 17(1):68. https://doi.org/10.1186/s13075-015-0591-8
  • Bolin AP, Guerra BA, Nascimento SJ, Otton R. Changes in lymphocyte oxidant/antioxidant parameters after carbonyl and antioxidant exposure. Int Immunopharmacol. 2012; 14(4):690-7. https://doi.org/10.1016/j.intimp.2012.10.003
  • Salem S, Leghouchi E, Soulimani R, Bouayed J. Reduction of paw edema and liver oxidative stress in carrageenan-induced acute inflammation by Lobaria pulmonaria and Parmelia caperata, lichen species, in mice. Int J Vitam Nutr Res. 2019; 1-9. https://doi.org/10.1024/0300-9831/a000620
  • Di Paola R, Mazzon E, Muià C, Genovese T, Menegazzi M, Zaffini R, et al. Green tea polyphenol extract attenuates lung injury in experimental model of carrageenan-induced pleurisy in mice. Respir Res. 2005; 6(1):66. https://doi.org/10.1186/1465-9921-6-66
  • Yermak IM, Barabanova AO, Aminin DL, Davydova VN, Sokolova EV, Solov'eva TF, et al. Effects of structural peculiarities of carrageenans on their immunomodulatory and anticoagulant activities. Carbohydr Polym. 2012; 87:713-720. https://doi.org/10.1016/j.carbpol.2011.08.053
  • Sokolova EV, Menzorova NI, Davydova VN, Kuz'mich AS, Kravchenko AO, Mishchenko NP, et al. Effects of carrageenans on biological properties of Echinochrome. Mar Drugs. 2018; 16(11):419. https://doi.org/10.3390/md16110419
  • Sokolova EV, Karetin Y, Davydova VN, Byankina AO, Kalitnik AA, Bogdanovich LN, et al. Carrageenans effect on neutrophils alone and in combination with LPS in vitro. J Biomed Mater Res A. 2016; 104(7):1603-9. https://doi.org/10.1002/jbm.a.35693
  • Wu W, Zhen Z, Niu T, Zhu X, Gao Y, Yan J, et al. κ-Carrageenan enhances lipopolysaccharide-induced interleukin-8 secretion by stimulating the Bcl10-NF-κB pathway in HT-29 cells and aggravates C. freundii-induced inflammation in mice. Mediators Inflamm. 2017; 2017:8634865. https://doi.org/10.1155/2017/8634865