Skin morphologу of patients with systemic scleroderma after autologous bone marrow stem cells transplantation

Морфология кожи больных системной склеродермией при трансплантации аутологичных клеток костного мозга
Galina Fedotovskikh 1, Galiya Shaimardanova 1, Manarbek Askarov 1, Aigerim Smagulova 1
More Detail
1 «National Scientific Medical Research Center» JSC, Astana, Kazakhstan
J CLIN MED KAZ, Volume 3, Issue 37, pp. 37-42.
OPEN ACCESS 3116 Views 2220 Downloads
Download Full Text (PDF)

ABSTRACT

The Aim: To evaluate a morphological pictures of patients skin with systemic scleroderma (SSc) before and after of autologous hematopoietic fraction of bone marrow cells (SCs) transplantation.
Methods: The skin biopsy from 15 patients were studied before and in 3 months after SCs transplantation. The samples were stained with hematoxiylin and eosin and Masson-trihrom. Immunochemical study was performed using the CD 31, 3, 19 markers. The electron microscopy ( EM) has been done by conventional method and embedded in Epon. The semithin section were stained with methylene blue, azure- 2 and basic fuchsin. Ultrathin section treated by uranylacetate and leadcitrate in accordance with Reynolds and examined with an EM Libra 120 (Carl Zeiss).
Results: There were an epidermis atrophy, fibrosis of the connective tissue of derma and microvascular pathology in the skin samples before treatment. Nondifferentiated cells,active  fibroblast and miofibroblast are presented there. In three  month after treatment there were  observed significant changes, namely: decreasing of skin  density from 12,9 to 8,7, decreasing  of the collagen deposit, neovascularisation, telocytes migration, activating  of macrophages and fibroclasts which take part in remodeling of fibrous tissues. Restoration of epidermis, hair follicles and sweat glands.
Conclusion: Transplantation of SCs cells make a contributions of fibrose reduction, angiogenesis and skin epithelium remodeling.
Keywords: scleroderma, skin, cell transplantation, morphology

CITATION

Fedotovskikh G, Shaimardanova G, Askarov M, Smagulova A. Skin morphologу of patients with systemic scleroderma after autologous bone marrow stem cells transplantation. Journal of Clinical Medicine of Kazakhstan. 2015;3(37):37-42.

REFERENCES

  • Nevskaja T.A., Anan’eva L.P., Guseva N.G. V-kletochnye narushenija pri sistemnoj sklerodermii: vzaimosvjaz’ s fibrozom i vaskulopatiej, novye podhody k lecheniju (B-cell disorders in systemic sclerosis : relationship with fibrosis and vasculopathy, new approaches to treatment), Zhurnal nauchno–prakticheskaja revmatologija, 2009, No. 1, pp.43 – 60.
  • Guseva N.G. Sistemnaja sklerodermija – mul’tidisciplinarnaja problema (Systemic scleroderma - a multidisciplinary problem), Zhurnal nauchno – prakticheskaja revmatologija, 2011, No. 2, pp.10 – 14.
  • Koneva O.A., Anan’eva L.P., Ovsjannikova O.B. i dr. Issledovanie jeffektivnosti i bezopasnosti terapii mikofenolata mofetilom u bol’nyh sistemnoj sklerodermiej (Study the efficacy and safety of mycophenolate mofetil therapy in patients with systemic sclerosis), Zhurnal nauchno – prakticheskaja revmatologija, 2011, No.4, pp.16 – 23.
  • Alekperov R.T., Ljubimova E.G. Statiny pri sistemnoj sklerodermii (Statins in systemic sclerosis), Zhurnal medicinskij sovet, 2008, No.5-6, pp. 1-2.
  • Anan’eva L.P., Desinova O.V., Koneva O.A. i dr. Lechenie retuksimabom intersticial’nogo porazhenija legkih pri sistemnoj sklerodermii (Treatment retuksimabom interstitial lung disease in systemic sclerosis), Zhurnal nauchno – prakticheskaja revmatologija, 2013, No.5, pp.514 – 523.
  • Novik A.A. Transplantacija stvolovyh krovetvornyh kletok pri autoimmunnyh zabolevanijah (Stem cell transplantation in autoimmune diseases), Zhurnal vest.Ros.voenno/med.akad, 2002, T.7, No. 1, pp.98 – 102.
  • Alekperov R.T., Anan’eva L.P. Transplantacija autologichnyh gemopojeticheskih stvolovyh kletok pri sistemnoj sklerodermii (Transplantation of autologous hematopoietic stem cells in systemic sclerosis), Zhurnal nauchno – prakticheskaja revmatologija, 2012, No.53(4), pp. 67–72.
  • J.M.van Laar, Tyndall.A. Adult stem cell in the treatment of autoimmune diseases, Rheumatology, 2006, Vol. 45, pp.1187– 1193.
  • Kissin E.Y., Merkel P.A., Lafyatis R. Myofibroblasts and hyalinized collagen as markers of skin disease in systemic sclerosis, Arthr Rheum, 2006, Vol. 54, pp.3655–60.
  • Preliminary criteria for the classification of systemic sclerosis (scleroderma). Subcommittee for scleroderma criteria of the American Rheumatism Association Diagnostic and Therapeutic Criteria Committee, After Rheum, 1980, Vol. 23, pp. 581-90.
  • Revmatologija: nacional’noe rukovodstvo (Rheumatology: national leadership), pod red. E.L. Nasonova, V.A. Nasonovoj, M.: GJeOTAR-Media, 2008, 720 p.
  • Santiago B., Galindo M., Rivero M., Pablos J–L. Decreased susceptibility to Fas-induced apoptosis of systemic sclerosis dermal fibroblasts, Arthritis Rheum, 2001, Vol. 44(7), pp.1667–1676.
  • Manole C.G., Cismasiu V., Gherghiceanu Mihaela, Popescu L.M., J Ctll Mol Med, 2011, Vol. 15, No.11, pp.2284-2296.
  • Teoreticheskie voprosy travmatologii i ortopedii (Theoretical questions of traumatology and orthopedics), Berchenko G.N. Moskva, 1990, pp.19 – 32.
  • Immunopatogenez v infekcionnom processe (Immunopatogenez in an infectious process), Beklemishev N.D., Coj I.G. «Ғylym» Alma-Ata, 1992, 335 p.