Systemic administration of autologous mononuclear precultured bone marrow stem cells in heart failure

Системное введение аутологичных мононуклеарных прекультивированных клеток костного мозга при сердечной недостаточности
Aliya Dzholdasbekova 1, Galina Fedotovskikh 1, Manarbek Askarov 1, Bayan Komsabakova 1, Ainur Baigenzhina 1, Argul Kairatova 1, Gulden Abylkassymova 1
More Detail
1 «National Scientific Medical Research Center» JSC, Astana, Kazakhstan
J CLIN MED KAZ, Volume 3, Issue 37, pp. 14-18.
OPEN ACCESS 3105 Views 2339 Downloads
Download Full Text (PDF)

ABSTRACT

The Aim: Heart failure (HF) is the major cause of death worldwide. Despite the available pharmacotherapy, prognosis is usually harsh. Here we assessed the safety and efficacy of autologous bone marrow-derivedmononuclear cell (BMMC)retransplantation in HF (left ventricular ejection fraction, LVEF <45%) at one-year follow-up.
Methods: A whole number of 104 patients were included to the study (male=84 and female=20), age 27 untill 65 with Heart failure. All patients underwent standard examination (12-lead ECG, echocardiography, 6-minute walking test and laboratory tests).
Results: After systemic BMMC retransplantation, EF was shown to rise at 12 months for both DCMP and ISCM (32,1+/-1,3 vs 41,3+/-1,6% and 32,2+/-1,2% vs 39,3+2,6%, respectively; p-value<0,005). ProBNP recognized as an early marker of HF tend to reduce sufficiently by 3 monthsand continued to recover at one-year follow-up for both diseases (3266,+/-344,4 vs 361+/-35,9 ng/ml; p-value<0,0001 and 4618+/-267 vs 286+/-35,9 ng/ml; p-value<0,0001 for ICMP and DCMP, respectively).
Conclusion: Our results suggest BMMC transplantation is safe and well tolerated. BMMC infusion has improved systolic myocardial function, and a consequence has delayed the progression of HF, which is confirmed by recovering proBNP. Moreover, BMMC transplantation might favor by preliminarycell cultivation due to enhanced mitochondrial function.
Keywords: stem cells, bone marrow, ischemic heart disease, dilated cardiomyopathy, interleukins

CITATION

Dzholdasbekova A, Fedotovskikh G, Askarov M, Komsabakova B, Baigenzhina A, Kairatova A, et al. Systemic administration of autologous mononuclear precultured bone marrow stem cells in heart failure. Journal of Clinical Medicine of Kazakhstan. 2015;3(37):14-8.

REFERENCES

  • Repin B.S., Suhih G.T. Medical Stem Biology, M., RAMS:М., 1998, pp.56-58.
  • Maslov L.N., Rjbov N.N., Sazonova C.N.Cellular transplantation is in treatment of myocardial infarction: problems and prospects, Vestn.transplantol. and artificial organs, 2003, Vol.4, рр.78-86.
  • Maslov C.N., Rjbov N.N., Sazonova C.N. Regeneration of myocardium// Successes of physiological sciences.- 2004.-Vol.3.- рр.50-60.
  • Karpov V.C., Markov V.A., Rjbov N.N. Theoretical aspects of cellular technologies in a cardiology and results of own research, Collection of labours to the 80year of academician E.I.Chazova, 2009, рр.104-115.
  • Stepanov O.D., Chadin A.V., Masutin A.G. and all. Miosin activating proteinkinases are possible regulators of not myscle miosin in the developing heart of man, Bulletin of eksperimental biologii and medicines, 2011, Vol.152 No.152, pp.158-161.
  • Humphrey C, Pittman F. A simple methylene blue-azure II-basic fuchsin stain for epoxy-embedded tissue sections, Stain Technol, 1974, No.49, pp.9–14.
  • Sekamova S.N., Beketova T.P. About the functional value of dark and light cages, Archive of pathology, 1975, No. 5, pp.57-64.
  • Klembovski A.N., Suhorukov V.S. Problem of power disfunction of cages at pathology of man (pathogeny and correction), Ann. of the Russian academy of natural sciences, 2007, No.4, pp.62-69.
  • Postnov U.V. About the energydependent link of pathogeny of chronic gipertenzii, Archiv pathologies, 2009, No.4, pp.3-11.
  • Rector T.S., Francis G.S., Cohn J.N. Patients self-assessment of their congestive heart failure. Part 1: Patient perceived dysfunction and its poor correlation with maximal exercise tests, Heart Failure, 1987, Vol.3, pp. 192-196.
  • Rector T.S., Kubo S.H., Cohn J.N. Patients self-assessament of their congestive heart failure. Part 2: Content, realibility and validity of a new measure, the Minnessota Living with Heart failure Questionnaire, Heart Failure, 1987, Vol. 3, pp.198-209.
  • FDP. Metabolic support at the extreme states. General description of preparation. Consortium «Medexport Italiya». «Fisiofarma», 2006, 12 p.
  • Karra R.S., Vemullapalli C., Dong C. et al.Stem cells of aging donors-insufficient capacity to repair causes progression of atherosclerosis in the recipient: molecular evidence for arterial repair in atherosclerosis, Proc.Nat.Acad.Sci.USA, 2005, Vol.102, pp.16789-16794.
  • Iso Y., Spees J.L., Serano C.et al. Multipotent human stromal cells improve cardiac function after myocardial infarction in mice without long-term engraftment, Biochem. Biophys. Res. Commun, 2007, No.354(3), pp.700-6.
  • Lee R.H., Pulin A.A., Seo M.J. et al. Intravenous hMSCs improve myocardial infarction in mice because cells embolized in lung are activated to secrete the anti inflommatory protein TSG-6, Cell.Stem.Cell, 2009, No.5(1), pp.54-63.