The cytokine status in experimental geliotrin hepatitis

Состояние цитокинового статуса при экспериментальном гелиотриновом гепатите
Rikhsi Sabirova 1, Munojat Kulmanova 1
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1 Tashkent Medical Academy, Tashkent, Republic of Uzbekistan
J CLIN MED KAZ, Volume 4, Issue 34, pp. 46-48.
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ABSTRACT

The aim. To study cytokines in experimental hepatitis depending on the timing of study.
Methods. Experiments were carried on 60 white male rats weighing 100-120 g. Heliotrinic hepatitis (HH) was reproduced by oral injection of heliotrin solution, acidified with hydrochloric acid (pH 7.0) at the rate of 50 mg / kg (5 mg heliotrin per 100g) weight once a week for 42 days. Then animals were decapitated on the 60th, 90th and 120th days of the experiment. Interleukin (IL-1b, IL-2, IL-6) and tumor necrosis factor-alpha (TNF-α) were determined on ELISA analyzer of «Stat-Fax» (USA) firm using a set of test kits produced by LTD «Cytokine» (St. Petersburg).
Results. In the dynamics of the development experimental heliotrinic hepatitis set of induction of cytokines IL-1b, IL-2, IL-6, the most increase these cytokines in comparison with intact animals was on the 90th day of the experiment.
Conclusion. Immune dysfunction plays an important role in progression of pathophysiological processes, in course, severity and outcome of the disease in heliotrinic hepatitis.
Keywords: hepatitis, cytokines, heliotrinic

CITATION

Sabirova R, Kulmanova M. The cytokine status in experimental geliotrin hepatitis. Journal of Clinical Medicine of Kazakhstan. 2014;4(34):46-8.

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