The effect of laparoscopy and laparotomy on hemostasis in experimental colorectal cancer surgery in rats

Eyüp Murat Yılmaz 1 * , Erdem Barış Cartı 1, Çiğdem Yenisey 2, Mahir Kırnap 1, Muhammed Çağrı Coşkun 1, Ahmet Ender Demirkıran 1, Nesibe Kahraman Çetin 3
More Detail
1 General Surgery Department, Aydın Adnan Menderes Üniversity, Aydin, Turkey
2 Biochemistry Department, Aydın Adnan Menderes Üniversity, Aydin, Turkey
3 Pathology Department, Aydın Adnan Menderes Üniversity, Aydin, Turkey
* Corresponding Author
J CLIN MED KAZ, Volume 6, Issue 60, pp. 83-87. https://doi.org/10.23950/jcmk/9269
OPEN ACCESS 1765 Views 1530 Downloads
Download Full Text (PDF)

ABSTRACT

Background: Nowadays, it is claimed that advanced laparoscopic methods are minimally invasive, provide quick recovery of normal functions in patients and cause less stress in the organism compared to similar open surgery techniques. There is only limited research on 8-OHdG and 8-OHG levels, which are the markers of oxidative stress in both laparoscopic and open surgery. In this experimental study, we investigated the stress level caused by the laparotomic and laparoscopic colon resections in rats with induced colorectal cancer model.
Material and methods: In this study, colon cancer was induced with subcutaneous dimethylhydrazine (DMH) (20mg/kg) in 30 Sprague-Dawley male rats. After 12 weeks, rats were divided into three groups: Group 1 (control group; n=10), Group 2 (laparotomy group; n=10), and Group 2 (laparoscopy group; n=10). Afterward, blood and lung tissue samples were obtained, and 3-NT, 4-HNE, OHdG, PGF2, and NO levels were measured.
Results: There was a statistically significant difference between the groups in terms of 3-NT (ng/ml), which is a biomarker of oxidative stress (p<0.01). After obtaining blood samples from rats, DNA and RNA were isolated, and a statistically significant difference was found between the laparotomy and laparoscopy groups in terms of 8-OHdG and 8-OHG molecules (p<0.01).
Conclusion: Laparoscopy, which initiated a new era in the scientific field, provided various advantages over laparotomy and widely used in colorectal surgery. In addition, it was demonstrated that laparoscopic surgery caused less oxidative stress, had advantageous effects on the antioxidant defense system, and thus superior to laparotomy.
Keywords: laparoscopic surgery, colorectal cancer, laparotomy, 3-NT, 8-OHdG

CITATION

Yılmaz EM, Cartı EB, Yenisey Ç, Kırnap M, Coşkun MÇ, Demirkıran AE, et al. The effect of laparoscopy and laparotomy on hemostasis in experimental colorectal cancer surgery in rats. J Clin Med Kaz. 2020;6(60):83-7. https://doi.org/10.23950/jcmk/9269

REFERENCES

  • Kopp I, Bauhofer A, Koller M. Understanding quality of life in patients with colorectal cancer: comparison of data from a randomised controlled trial, a population based cohort study and the norm reference population. Inflamm Res. 2004; 53:S130-5. https://doi.org/10.1007/s00011-004-0361-6
  • King PM, Blazeby JM, Ewings P, Kennedy RH. Detailed evaluation of functional recovery following laparoscopic or open surgery for colorectal cancer within an enhanced recovery programme. Int J Colorectal Dis. 2008; 23:795-800 https://doi.org/10.1007/s00384-008-0478-0
  • Pappas-Gogos G, Tellis C, Lasithiotakis K, Tselepis AD, Tsimogiannis K, Tsimoyiannis E, Chalkiadakis G et al. Oxidative stress markers in laparoscopic versus open colectomy for cancer: a double-blind randomized study. Surg Endosc. 2013; 27(7):2357-2365. https://doi.org/10.1007/s00464-013-2788-8
  • Aspinen S, Harju J, Juvonen P, Selander T, Kokki H, Pulkki K et al. Scand J. Theplasma 8-OHdG levels and oxidative stress following cholecystectomy: a randomised multicentre study of patients with mini laparotomy cholecystectomy versus laparoscopic cholecystectomy. Scand j Gastroenterol 2016; 51(12):1507-1511 https://doi.org/10.1080/00365521.2016.1208270
  • Zulfikaroglu B, Koc M, Soran A, Isman FK, Cinel I. Evaluation of oxidative stress in laparoscopic cholecystectomy. SurgToday. 2002; 32(10): 869-874. https://doi.org/10.1007/s005950200169
  • Braga M, Frasson M, Vignali A, Zuliani W, Civelli V, et al. Laparoscopic vs. open colectomy in cancer patients: long-term complications, quality of life, and survival. Dis Colon Rectum. 2005; 48:2217-23 https://doi.org/10.1007/s10350-005-0185-7
  • Glantzounis GK, Tselepis AD, Tambaki AP, Trikalinos TA, Manataki AD, Galaris DA et al.. Laparoscopic surgery-induced changes in oxidative stress markers in human plasma. Surg Endosc. 200; 15(11):1315-1319. https://doi.org/10.1007/s00464-001-0034-2
  • Stipancic I, Zarkovic N, Servis D, Sabolović S, Tatzber F, Busic Z. Oxidative stress markers afterlaparoscopicandopencholecystectomy. JLaparoendoscAdvSurgTech A. 2005; 15(4):347-352. https://doi.org/10.1089/lap.2005.15.347
  • Matsuda T, Fujita H, Kunimoto Y, Kimura T, OginoK.Analysis of Recurrent Casesafter Laparoscopic Surgery for Colorectal Cancer. Hepatogastroenterology. 2014; 61(132):1028-1032.
  • Arezzo A, Passera R, Ferri V, Gonella F, Cirocchi R, Morino M. Laparoscopic right colectomy reduces short-term mortality and morbidity. Results of a systematic review and meta-analysis. Int J Colorectal Dis. 2015; 30:1457-1472. https://doi.org/10.1007/s00384-015-2304-9
  • Arung W, Drion P, Cheramy JP, Honore P, Meurisse M, Defraigne JO et al. Intraperitonealadhesionsafteropenorlaparoscopicabdominalprocedure: an experimentalstudy in therat. J LaparoendoscAdvSurgTech A. 2012; 22(7):651-657. https://doi.org/10.1089/lap.2012.0102
  • Mynbaev OA, Eliseeva MY, Kalzhanov ZR, Lyutova L, Pismensky SV, Tinelli A, et al. Surgical trauma and CO2-insufflation impact on adhesion formation in parietal and visceral peritoneal lesions. Int J Clin Exp Med. 2013; 6(3):153-165.
  • Jurczok A, Schneider A, FornaraP.Inhibition of tumor implantationafter laparoscopy byspecificoligopeptides: a novelapproachtoadjuvantintraperitonealtherapytoprevent tumor implantation in an animal model. Eur Urol. 2007; 52(2):590-595. https://doi.org/10.1016/j.eururo.2006.10.057
  • Kumar AS, Lee SW. Laparoscopy in Colorectal Surgery. Surg Clin Nort Am. 2013; 93:217-230. https://doi.org/10.1016/j.suc.2012.09.006