The molecular determination of emm genotypes in non-group a beta-hemolytic streptococci isolated from clinical samples

Suna Kızılyıldırım 1 * , Cansu Önlen Güneri 2, Fatih Köksal 3
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1 Depertmant of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Süleyman Demirel, 32260, Isparta, Turkey.
2 Department of Medical Microbiology, Gulhane Vocational School of Health Services, University of Sağlık Bilimleri, 06010, Ankara, Turkey.
3 Department of Medical Microbiology, Medical Faculty, Cukurova University, 01380, Adana, Turkey.
* Corresponding Author
J CLIN MED KAZ, Volume 18, Issue 4, pp. 64-68. https://doi.org/10.23950/jcmk/11045
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ABSTRACT

Objective: M protein is an important marker in epidemiological and phylogenetic surveillance of Beta-Hemolytic Streptococci.
The aim of this study is to determine emm types and their distribution among the GCS/GGS strains isolated from clinical samples.
Material and methods: The study includes 98 β-hemolytic streptococcus strains isolated from clinical samples in Çukurova University/Balcalı Hospital. 67 of these isolates, defined as serologic, of which 56 were identified as Streptococcus dysgalactiae subsp. equisimilis, 8 as S. anginosus and 3 as S.equi subsp.zooepedimicus, have been included in the study. These strains were also confirmed by the Vitek 2GP-ID system. These isolates were confirmed by the polymerase chain reaction (PCR) method with primers based on groESL sequences. PCR-sequence analysis method developed by the Centers for Disease Control (CDC) was used for typing according to emm polymorphism.
Results: 56/67 (83.6%) isolates in which emm-PCR was determined as positive were typed by sequence analysis. The findings were identified using the CDC database, and the most common type was determined to be stG485 (51.85%), stG643 (12.96%) and stG6 (9.25%).
Conclusion: Consequently, GCS/GGS should be treated more seriously in our country as in the whole world.
Keywords: emm, PCR, GCS, GGS.

CITATION

Kızılyıldırım S, Önlen Güneri C, Köksal F. The molecular determination of emm genotypes in non-group a beta-hemolytic streptococci isolated from clinical samples. J CLIN MED KAZ. 2021;18(4):64-8. https://doi.org/10.23950/jcmk/11045

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