Ultrastructure of bone marrow cells in patients with autoimmune pathologies
Ультраструктура клеток костного мозга больных с аутоиммунной патологий
Galina Fedotovskikh 1, Galiya Shaimardanova 2, Manarbek Askarov 3, Sapargul Marat 1, Tatyana Yezhelenko 1
More Detail
1 Scientific and Research Laboratory, National Scientific Medical Center, Astana, Republic of Kazakhstan
2 Scientific Research Management Department, National Scientific Medical Center, Astana, Republic of Kazakhstan
3 Center of Cellular Technologies and Transplantation, National Scientific Medical Center, Astana, Republic of Kazakhstan
J CLIN MED KAZ, Volume 1, Issue 47, pp. 49-52.
https://doi.org/10.23950/1812-2892-JCMK-00536
OPEN ACCESS
3072 Views
2311 Downloads
ABSTRACT
Objective: To determine the morpho-functional state of bone marrow autologous cells at the ultrastructural level.
Material and Methods: For the electron microscopic examination, bone marrow cells taken from patients with autoimmune diseases were fixed in a 2.5% solution of glutaraldehyde with post fixation in a 1% solution of osmium tetroxide, was performed according to the conventional method and enclosed in epon. Ultrathin sections were prepared on a Leica ultramicrotome, contrasted with lead citrate by Reynolds, and examined in an electron microscope called Libra 120 (C. Zeiss).
Results: Morpho-functional state of autologous bone marrow cells in severe chronic pathology was characterized by mitochondrial dysfunction and signs of insufficiency of microvesicular secretory function.
Conclusion: Precultivation of bone marrow cells of patients with severe autoimmune diseases restored the energetic function of mitochondria, increased the secretory activity of endosomes.
CITATION
Fedotovskikh G, Shaimardanova G, Askarov M, Marat S, Yezhelenko T. Ultrastructure of bone marrow cells in patients with autoimmune pathologies. Journal of Clinical Medicine of Kazakhstan. 2018;1(47):49-52.
https://doi.org/10.23950/1812-2892-JCMK-00536
REFERENCES
- Sekamova S.M., Beketova T.P. O funkcional’nom znachenii temnyh i svetlyh kletok (About the functional significance of dark and light cells) [in Russian]. Arkhiv patologii.1975;5
- Klembovskii A.I., Sukhorukov V.S. Problema jenergeticheskoj disfunkcii kletok pri patologii cheloveka (patogenez i korrekcija) (The problem of energy dysfunction of cells in human pathology (pathogenesis and correction) [in Russian]. Vestnik Rossiiskoi akademii estestvennykh nauk. 2007; 62,69
- Postnov Y.V. O jenergozavisimom zvene patogeneza hronicheskoj gipertenzii (About the energy-dependent link of chronic hypertension pathogenesis)[in Russian]. Arkhiv patologii. 2009;3,11
- Stepanova O.I., Kasinskaya N.V., Baranova O.V., et al. Metod kul’tivacii i fenotipicheskaja harakteristika gemopojeticheskih kletok kostnogo mozga (mononuklearnoj frakcii) ot donorov s genom zelenogo belka (Method of cultivation and phenotypic characteristic of hematopoietic bone marrow cells (mononuclear fraction) from donors with the gene of green protein [in Russian]. Biomeditsina. 2013;91,94